Screening for transformed human mesenchymal stromal cells with tumorigenic potential

Spontaneous transformation of human mesenchymal stem/stromal cells has been observed during long-term expansion in cell culture, although it is rare. Engrafting these transformed cells into immunodeficient mice leads to the formation of solid tumors. Using high-throughput profiling methods, a panel of RNA molecules was identified as potential biomarkers for screening for these transformed cells in cell culture.