Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of the most significant developments in optics. Due to the wave nature of light, even the best lens cannot produce a light spot smaller than 250 nanometers in diameter. All molecules within this bright spot are illuminated at the same time, light up together, and therefore, appear inseparable as a blurred whole.
Super-resolution microscopy achieves nanometer resolution without traditional ON/OFF switching
Phys.org - 27 Feb 2025 23:14
Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of the most significant developments in optics. Due to the wave nature of light, even the best lens cannot produce a light spot smaller than 250 nanometers in diameter. All molecules within this bright spot are illuminated at the same time, light up together, and therefore, appear inseparable as a blurred whole.
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